This is the second article in this series discussing HPLC oligonucleotide separations. This article will discuss the separation of oligonucleotides and dsDNAs using a wide-pore, polymer based reversed ...

Figure 1: Schematic representation of the continuous FFE apparatus coupled off-line to RP-HPLC. Hydroxypropyl methyl cellulose (HPMC) stock solution, 2% (wt/vol; ∼86 K nominal molecular weight; ...

Given the nature of biomolecules, such as amino acids and peptides, many HPLC reversed phase bio-separations often require ion-pairing reagents, such as Trifluoroacetic acid (TFA), in combination with ...

Evaluation of: Stephanowitz H, Lange S, Lang D et al. Improved two-dimensional reversed phase–reversed phase LC-MS/MS approach for identification of peptide–protein interactions. J. Proteome Res. 11(2 ...

This paper describes a unique Prep-rP-HPLC technique that uses a C-18/C-8 derivatized silica coated with a hydrophobic quaternary ammonium salt or quaternary phosphonium salt that acts as an ...

Operating HPLC at higher than ambient temperatures can improve peak shapes and enable faster run times. Preheating the mobile phase is significant in high temperature liquid chromatography as the ...

The term liquid chromatography (LC) refers to a class of physicochemical methods which are used to separate and quantify compounds in a sample. LC is made up of a liquid mobile phase, which carries ...

Normal phase HPLC is a valuable technique for the separation of polar and hydrophilic compounds and for isomer separation. Normal phase HPLC requires a polar stationary phase combined with non or ...

Announcing a new article publication for BIO Integration journal. Ellagic acid (EA) is a natural polyphenolic compound recognized for bioactive and pharmacologic properties that is found abundantly in ...

What is Hydrophilic Interaction Chromatography? HILIC uses a polar stationary phase, such as silica or polar bonded phase, and the mobile phase consists of 5- 40% water that is combined with a less ...