Lysis buffer composition and protein lysate preparation is crucial for the success of RPPA. Discuss with the core director before preparing your lysates. View detailed protocols for lysate preparation ...
Cell or tissue lysates are arrayed on nitrocellulose-coated slides (Grace Bio-labs, Bend, OR) in triplicate using the Aushon 2470 Arrayer (Aushon BioSystems, Billerica, MA) (technical replicates).
Normalization computes a ratio between the response of an individual neuron and the summed activity of a pool of neurons. The normalization model was developed to explain responses in the primary ...
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