This also enabled the international research team to study the interaction of the fungus with immune cells. In the current ...

1| Collect the cells to be transfected from cultures in the mid- to late-logarithmic phase of growth. Use either a rubber policeman or trypsin to release adherent cells. Centrifuge at 500g at 4 ...

Scrape each plate of cells into 1 ml of ice-cold EBC lysis buffer ... 9| Digest the protein with trypsin while it is still in the gel, and electroelute the peptides. (i) Remove the gel slice ...