Cytospins were incubated with saponin before the first and second antibody. A goat anti-rabbit antibody conjugated to peroxidase (1:400 dilution) was used as a second step. The peroxidase reaction was ...

The reaction mechanism for the reduction of hydrogen peroxide by N,N-dimethylbenzylamine diselenide, its selenol analogue, and the charged analogues of the diselenide and selenol are elucidated using ...

Recent advancements in g-C3N4 photocatalysts enhance H2O2 production and pollutant degradation under visible light through ...

Slices were washed with ABC Elite Kit and peroxidase reaction was visualized by 3’3-diaminobenzidine. After washing, ... After stopping the reaction with 0.2 M H2SO4 the optical density was detected ...

After washing with PBS, secondary antibodies biotinylated anti-mouse IgG (1:250 dilution, BA-9200, Vector Laboratories, California, United States) and biotinylated anti-rabbit IgG (1:250 dilution, ...

After the enzymic reactions, the generated hydrogen peroxide (H2O2) was oxidized by V-porous Co3O4 to produce hydroxy radicals (.OH), inducing ABTS and TMB to generate colored products. The generated ...

To prevent endogenous peroxidase reactions, the samples were pretreated with 0.3% H 2 O 2 in cold methanol for 30 minutes. Finally, 0.1 mg/mL of 3, 3′-diaminobenzidine tetrahydrochloride was applied ...