For whole liver lysate, frozen liver was homogenized in a glass homogenization tube using a mechanical pestle grinder with homogenization buffer (50 mM Tris pH 7.6, 5 mM EDTA, 150 mM NaCl, 0.1% SDS, 0 ...

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All experiments described in this article were conducted in Lysogeny Broth, with appropriate antibiotics (100 μg/mL Ampicillin, 10 μg/mL Tetracycline, and 50 μg/mL Kanamycin). The experiments were ...

The tissue was submerged in ∼25 ml of 4% paraformaldehyde (PFA) in 1x TBS and placed at 4°C on a rocker at low speed for ∼16-18 hrs. The tissue was washed 3 times with 1x PBS before incubation in 30% ...

For men in their 40s and 50s: A PSA score greater than 2.5 ng/ml is considered abnormal. The median PSA for this age range is 0.6 to 0.7 ng/ml. For men in their 60s: A PSA score greater than 4.0 ng/ml ...