Carboxylesterase activity assay. The culture medium of HeLa cells transfected with pF39-sCE2 or pSectag2 were concentrated 10 times using Protein-Concentrate kit (Millipore, Billerica, MA).

Carboxylesterase (CES): Enzymes that catalyse the hydrolysis of ester, thioester, and amide bonds, affecting drug activation and detoxification. Prodrug: An inactive compound that is metabolically ...

The purpose of this study was to examine the role of carboxylesterase and β-glucuronidase in the in vitro production of SN-38. Methods: The production of SN-38 from CPT-11 and SN-38G (19.2, 9.6 and ...