Proteolysis targeting chimera (PROTAC) degraders are typically bifunctional with one E3 ligase ligand connected to one target protein ligand via a linker. While augmented valency has been shown with ...

Opti-MEM media was exchanged with fresh media 6 h after transfection. The supernatant containing virus was collected at 24- and 48 h post-transfection and pooled together. The virus was cleared by ...

Thirty picomoles of FoxO1 or control siRNA was diluted in 150 μL Opti-MEM Medium, then mixed with 150 μL Opti-MEM Medium containing 9 μL Lipofectamine RNAiMAX. The mixture was incubated in RT for 5 ...

The supernatant was filtered through a 0.45 µm sterile filter (Merck/Millipore, Darmstadt, Germany) and first applied to an Amicon Ultra-15, MWCO 100 kDa, centrifugal filter unit (Merck/Millipore, ...

The siRNAs were diluted to 400nM in OPTI-MEM (Gibco, California, USA; Cat# 31985-062). GV-stage oocytes were transferred to the siRNA-containing Opti-MEM medium. Then oocytes and medium (total volume ...

Briefly, the miRNA mimics were reconstituted into a final 40 μM stock solution, 8 μL of which was diluted in 1 mL Opti-MEM medium and mixed with lipofectamine RNAiMAX reagent (8 μL diluted in 1 mL ...

Eight hours following transfection, Opti-MEM media was replaced with fresh DMEM media. One week later, cells were trypsinized and individual cells were seeded in a 96-well plate. A positive single ...

Briefly, for each plate 25 µl of Lipofectamine 3000 were diluted in 1.5 ml of Opti-MEM Medium (Gibco, Thermo Fisher Scientific, Waltham, MS, USA). Separately, master mix was prepared by diluting DNA ...

After the collagen polymerized, aortic rings were fed with 150 μL Opti-MEM containing 2.5% FBS and 40 ng/mL VEGF and incubated at 37 °C with 5% CO 2. After 4 d of incubation, the media were replaced.

Remove all of the growth medium, as serum will lower the transfection efficiency of the CPP conjugates in the serum-free OPTI-MEM. Remove the medium from one side of the well by tilting the plate ...