Dec 12, 2003 · Coexpression of the Ebola virus glycoprotein (GP) and matrix protein (VP40) in mammalian cells results in spontaneous production and release of virus-like particles (VLPs) that resemble the distinctively filamentous infectious virions.

Aug 10, 2022 · Ebola Virus are generally approximately 80 nm in diameter, 970 nm long. They are cylindrical/tubular, and contain viral envelope, matrix, and nucleocapsid components.

Mar 10, 2025 · Here, the authors use cryo-electron microscopy to structurally characterise the Ebola virus nucleocapsid within virus-like particles, uncovering how specific interactions regulate viral...

Using biochemical assays, it has been demonstrated that expression of Ebola virus VP40 alone in mammalian cells induced production of particles with a density similar to that of virions.

Mar 9, 2024 · An Ebola virus particle, also known as a virion, is made up of various components that allow it to infect and replicate within host cells. These components include genetic material, proteins, and a lipid envelope.

Here we have demonstrated that VP40 of Ebola virus, when it is expressed in the absence of other viral proteins, can induce the formation of membrane-encompassed particles, much in the manner of the matrix proteins of VSV, rabies virus, and …

Ebola virus encodes 7 structural genes that assemble to yield distinct filamentous viral particles. The EBOV-encoded major matrix protein VP40 traffics to the plasma membrane (PM), which leads to the formation and release of the virus-like particles (VLPs), even …

May 25, 2024 · Ebola virus (EBOV) matrix protein VP40 can assemble and bud as virus-like particles (VLPs) when expressed alone in mammalian cells. Nucleoprotein (NP) could be recruited to VLPs as inclusion...

We found that VP40 is indeed able to induce particle formation when it is expressed in mammalian cells, and this process appeared to rely on a conserved N-terminal PPXY motif, as mutation or loss of this motif resulted in markedly reduced particle formation.

Sep 13, 2021 · Using a transcription- and replication-competent VLP system, we showed that HERC5 inhibits EBOV virus-like particle (VLP) replication by depleting EBOV mRNAs. The HERC5 RCC1-like domain was necessary and sufficient for this inhibition and did not require zinc finger antiviral protein (ZAP).