However, it is also a subject that many flow cytometry users find difficult and confusing. Here we discuss some of the basics of spillover and compensation, tips for setting up compensation controls, and the …

Because the fluorophores used in flow cytometry emit photons of multiple energies and wavelengths, a mathematical method called compensation was developed to address the measurement of the …

In our experience, even if an experimental protocol is standardized beforehand with single tube run experiments, a “real time” compensation setting contributes significantly to the results.

Compensation in flow cytometry is the process of correcting for fluorescence spillover emissions. The detectors, or channels, in the instrument are designed to detect a very specific range of emissions.

The term “compensation,” as it applies to flow cytometric analysis, refers to the process of correcting for fluorescence spillover, i.e., removing the signal of any given fluorochrome from all detectors except …

Controls, compensation and calibration are all critical to obtaining accurate results in flow cytometric analysis. In this section you will find educational resources including application notes, videos, …

This article describes why compensation is required for flow cytometry and how to apply it. Please refer to the manufacturer’s instructions and software manual for a more detailed compensation procedure …

By applying good compensation controls such as antibody capture beads and accurate auto-matic compensation methods, which are now included in many flow cytometry software, we can increase …

Jun 24, 2020 · Compensation is used to ensure that the signal in a single detector is coming from the assigned fluorophore and not spillover from another fluorophore in the panel. Proper compensation is …

The use of antibody-labeled fluorochromes in flow cytometry assays requires a correction for the spillover of one fluorochrome signal into another detector. This correction is called compensation.