
Taq Polymerase - an overview | ScienceDirect Topics
Taq DNA Polymerase Taq DNA polymerase is an 832-amino acid protein with an inferred molecular weight of 93,920 and a specific activity of 292,000 units/ mg; optimal polymerization activity is …
Taq Polymerase - an overview | ScienceDirect Topics
Taq Polymerase is a type of DNA polymerase enzyme commonly used in real-time PCR due to its robust activity and speed. It is known for its residual activity at low temperatures, which can lead to …
A simple and efficient method for Taq DNA polymerase purification …
Apr 1, 2023 · Taq DNA polymerase from Thermus aquaticus (Taq) is a widely used enzyme in molecular biology research and clinical diagnostics because of its utility in DNA amplification and sequencing …
Taq Polymerase - an overview | ScienceDirect Topics
Taq DNA polymerase The Taq DNA polymerase used in the PCR is the DNA polymerase I enzyme of the bacterium Thermus aquaticus. This organism lives in hot springs, and many of its enzymes, …
Taq Polymerase - an overview | ScienceDirect Topics
Apr 3, 2012 · Taq polymerase is defined as the thermostable DNA polymerase I enzyme derived from the bacterium Thermus aquaticus, which is resistant to heat denaturation, making it suitable for …
High-level expression of codon-optimized Taq DNA polymerase under …
Sep 1, 2024 · Instead of full-length Taq polymerase, an N-terminal deletion of Taq polymerase was selected. To obtain a high-level expression, we attempted to optimize the codon by reducing the rare …
Development of a chemiluminescent detection method for absolute ...
Feb 15, 2025 · In our work, we developed a straightforward and precise quantitation approach for Taq DNA polymerase according to chemiluminescence detection of the consumption of dATP (CDC …
Bst polymerase — a humble relative of Taq polymerase
Jan 1, 2023 · The biochemical characteristics of the two enzymes and Taq-polymerase were also compared (Table 1). The specific activity, k cat, and processivity of Bst polymerase were significantly …
Trehalose and mannitol based lyoprotection of Taq DNA polymerase …
May 1, 2025 · The lyoprotectants showing enzyme protection or decent lyocake appearance for Taq were chosen as candidates for further lyoprotection research of Taq and 5 × HS-Taq Mix.
Isolation, Characterization, and Expression in - ScienceDirect
Apr 15, 1989 · The complete Taq DNA polymerase gene has 2499 base pairs. From the predicted 832-amino acid sequence of the Taq DNA polymerase gene, Taq DNA polymerase has significant …