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  1. Taq Polymerase - an overview | ScienceDirect Topics

    Taq DNA Polymerase Taq DNA polymerase is an 832-amino acid protein with an inferred molecular weight of 93,920 and a specific activity of 292,000 units/ mg; optimal polymerization activity is …

  2. Taq Polymerase - an overview | ScienceDirect Topics

    Taq Polymerase is a type of DNA polymerase enzyme commonly used in real-time PCR due to its robust activity and speed. It is known for its residual activity at low temperatures, which can lead to …

  3. A simple and efficient method for Taq DNA polymerase purification …

    Apr 1, 2023 · Taq DNA polymerase from Thermus aquaticus (Taq) is a widely used enzyme in molecular biology research and clinical diagnostics because of its utility in DNA amplification and sequencing …

  4. Taq Polymerase - an overview | ScienceDirect Topics

    Taq DNA polymerase The Taq DNA polymerase used in the PCR is the DNA polymerase I enzyme of the bacterium Thermus aquaticus. This organism lives in hot springs, and many of its enzymes, …

  5. Taq Polymerase - an overview | ScienceDirect Topics

    Apr 3, 2012 · Taq polymerase is defined as the thermostable DNA polymerase I enzyme derived from the bacterium Thermus aquaticus, which is resistant to heat denaturation, making it suitable for …

  6. High-level expression of codon-optimized Taq DNA polymerase under …

    Sep 1, 2024 · Instead of full-length Taq polymerase, an N-terminal deletion of Taq polymerase was selected. To obtain a high-level expression, we attempted to optimize the codon by reducing the rare …

  7. Development of a chemiluminescent detection method for absolute ...

    Feb 15, 2025 · In our work, we developed a straightforward and precise quantitation approach for Taq DNA polymerase according to chemiluminescence detection of the consumption of dATP (CDC …

  8. Bst polymerase — a humble relative of Taq polymerase

    Jan 1, 2023 · The biochemical characteristics of the two enzymes and Taq-polymerase were also compared (Table 1). The specific activity, k cat, and processivity of Bst polymerase were significantly …

  9. Trehalose and mannitol based lyoprotection of Taq DNA polymerase …

    May 1, 2025 · The lyoprotectants showing enzyme protection or decent lyocake appearance for Taq were chosen as candidates for further lyoprotection research of Taq and 5 × HS-Taq Mix.

  10. Isolation, Characterization, and Expression in - ScienceDirect

    Apr 15, 1989 · The complete Taq DNA polymerase gene has 2499 base pairs. From the predicted 832-amino acid sequence of the Taq DNA polymerase gene, Taq DNA polymerase has significant …